PYC Therapeutics

About

PYC Therapeutics is a drug-development company offering a new generation of RNA therapeutics to meet unmet need in disease. We have world-leading RNA targeted therapeutics design capabilities which are supported by our revolutionary delivery technology based on Cell Penetrating Peptides (CPPs) to overcome the ‘delivery challenge’ in genomic therapies. Our differentiated drug delivery capability and RNA drug design experts means we have the skills and means to discover and develop RNA-targeted therapeutics in a truly innovative way. We have three defined preclinical programs for Inherited Retinal Diseases, and a strong focus on Central Nervous System diseases. Guided by world-class scientists and business leaders who are at the forefront of innovation in their fields, our team has significant experience in the biotechnology sector.

https://pyctx.com/

https://au.linkedin.com/company/pyc-tx

https://twitter.com/pyctherapeutics

Project 

Project 1A:

 

The candidate will utilise their molecular biology knowledge and technical skills to:

 

a) develop a ddPCR assay designed to provide a more sensitive alternative to RT-PCR for improved detection of exon skipping in in vivo samples

b) develop a ddPCR assay designed to facilitate more accurate detection of protein isoform-specific transcripts with an aim to better match mRNA detection to protein quantification.

 

Activities will include:

  • Cellular harvest

  • mRNA synthesis

  • Performing QC

  • Designing and running ddPCRs and western blots

  • Performing data analysis and interpretation

  • Experimental documentation

  • Data presentation and communication

 

This work contributes towards the development of improved pharmacological assays with which to test the efficacy of therapeutic molecules for the treatment of incurable forms of blindness.

 

Project 1B:

 

PYC is committed to deliver therapeutic molecules into ocular cells to improve visually impaired patients’ lives. The candidate will utilise their research knowledge and technical skills to:

a) Develop and optimise assays designed to assess markers of disease that will improve the accuracy of pharmacological efficacy data interpretation

b) Investigate the expression of stress-associated markers in commercial and in-house derived retinal pigment epithelial cells

c) Integrate this data (see b) with quality control and functional data in order to determine a panel of patient-specific cellular parameters, compared to unaffected control cells.

 

Activities will include:

  • Performing QC, cellular harvest and processing, immunocytochemistry and microscopy

  • Designing and running flow cytometric assays 

  • Performing data analysis and interpretation

  • Experimental documentation

  • Data presentation and communication

 

This work will further our fundamental knowledge of the physiological effects of inherited retinal disease and strengthen the predictive power of in vitro pharmacological assays.

 

Project 2:

 

The candidate will develop computational pipelines to identify new therapeutic drug targets in the context of Ocular, CNS and/or Renal disorders. They will establish methods to evaluate approaches for gene expression modulation using data mining and other high-throughput analysis techniques.

 

Activities will include:

  • Working within a flexible AWS computational environment

  • Mining data from public databases and resources

  • Establishing discovery and analysis pipelines

  • Working with wet-lab scientists to confirm dry-lab findings

 

This work will grow the range of disease contexts within which we can apply our PPMO (Peptide-conjugated PMOs (Phosphorodiamidate morpholino oligomers) in the pursuit of therapeutic molecules for the treatment of incurable disorders.

Skills 

The ideal interns for Project 1A, 1B and 2 would be highly motivated and enthusiastic, with excellent attention to detail as well as data analysis, written and oral communication skills, and the ability to work productively within a dynamic team environment. Candidates must be highly organised with the ability to maintain detailed and accurate research records. A background in vision science, neuroscience/neurobiology or molecular biology will be an advantage, given the focus and commitment of PYC to deliver therapeutic molecules into ocular cells to improve visually impaired patients’ lives.

Project 1A:

 

Our ideal candidate for this placement is a meticulous research scientist, holding a Bachelor of Science (PhD) in a relevant discipline such as neuroscience, molecular biology, biochemistry, cell biology or immunology with at least 2 years of experience in molecular techniques, including RNA and protein extraction, RT-PCR and western blot. Experience in quantitative PCR techniques and biological assay development and optimisation is highly regarded, as is knowledge of ocular biology, genetics and inherited retinal disease.

Project 1B:

 

Our ideal candidate for this placement is a meticulous research scientist, holding a Bachelor of Science (PhD) in a relevant discipline such as neuroscience, molecular biology, biochemistry, cell biology or immunology with at least 2 years of experience in flow cytometric and immunocytochemical techniques, as well as molecular biology. Experience in biological assay development and optimisation is highly regarded, as is knowledge of ocular biology, genetics and inherited retinal disease.

 

Project 2:

 

Our ideal candidate will be proficient within a Linux environment and have strong programming skills, particularly with R, Perl and Python. They will be comfortable extracting data from public resources and analysing data from high throughput sequencing technologies such as RNA-Seq. Experience working within an AWS environment would be an advantage. 

Mode

  • Round 1 2022

  • 7 week placement 

  • Mixture of online and face-to-face engagement

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